Holographic photolysis of caged neurotransmitters
نویسندگان
چکیده
منابع مشابه
Four-dimensional multi-site photolysis of caged neurotransmitters
Neurons receive thousands of synaptic inputs that are distributed in space and time. The systematic study of how neurons process these inputs requires a technique to stimulate multiple yet highly targeted points of interest along the neuron's dendritic tree. Three-dimensional multi-focal patterns produced via holographic projection combined with two-photon photolysis of caged compounds can prov...
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Photolabile ‘caged’ compounds are biologically inert precursors of active molecules, which when irradiated with a pulse of light free the active species at its site of action (see Fig. 1). Speed is the principal advantage offered by these probes; photochemical reactions can be very fast, with release of the active species often complete within a millisecond. The method can thus be used to study...
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Caged compounds are biologically active molecules that have been rendered inert by the introduction of a photolabile protecting (caging) group to an important functional group. The intracellular photolysis of the caging group to reveal the active compound provides a powerful method for the intracellular release of biologically important molecules with exquisite temporal and spatial control. Thi...
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Irradiation of a mixture of 4-methoxyphenacyl-caged (S)-glutamate and 4,5-dimethoxy-2-nitrobenzyl-caged γ-amino butyric acid (GABA) on neurons, at ~260 nm, evokes selective photorelease of (S)-glutamate (Glu) whereas photolysis at 405 nm causes selective photorelease of GABA.
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Rapid concentration jumps of Ins(1,4,5)P3 or ATP were made inside Limulus ventral photoreceptors by flash photolysis of the parent caged compounds. In intact ventral photoreceptors, the photolysis flash evokes a maximum amplitude light-activated current; therefore, a procedure was developed for uncoupling phototransduction by blocking two of the initial reactions in the cascade, rhodopsin excit...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2008
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.1241